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After overnight centrifugation, the band containing the nozzles was collected using a gradient fractionator (Labconco Auto Densi-Flow), dialyzed against Tris buffer, and the nozzles were either collected by centrifugation (1 h at 366,000 g ) or further purified using 30 ml gradients of 10-40% sucrose (wt/wt)

But not everyone with a mutation in those genes will have autism the penetrance is very variable

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